ON CV-AAS DETERMINATION AND SPECIATION OF MERCURY IN WINE

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Published: Dec 15, 2023
Keywords:
mercury, wine, determination, speciation, cold vapor - atomic absorption spectrometry

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Krste Tašev
State Phytosanitary Laboratory, Bul. Aleksandar Makedonski bb, 1000 Skopje
Irina Karadjova
2Faculty of Chemistry, University of Sofia, J. Bourchier 1, 1164 Sofia
Ivan Boev
Faculty of Natural and Technical Sciences, Goce Delčev University, Blvd. Krste Misirkov 10A, 2001 Štip
Trajče Stafilov
Institute of Chemistry, Faculty of Science, St. Cyril and Methodius University, POB 162, 1000 Skopje, North Macedonia

Abstract

The possibilities of cold vapor - atomic absorption spectrometry (CV-AAS) for the determination and speciation of mercury in wine samples are critically discussed in the present study. In the first step, the direct determination of Hg using SnCl2 and NaBH4 as reducing agents is attempted. The influence of the type of reducing agent and its concentration, as well as the concentration of HCl on the absorbance signal of the different Hg species (Hg2+ and CH3Hg1+) is investigated. The results show that direct determination of both Hg species is possible: inorganic Hg can be selectively determined by using 0.04% NaBH4 and 1 mol/l HCl directly in an untreated wine sample; both Hg species are determined simultaneously by using 0.9% NaBH4 and 1 mol/l HCl again directly in an untreated wine sample. The recoveries obtained by using HCl at a concentration below 1 mol/l are lower than 85% which can be considered as evidence that the mercury in the wine sample is bound in some complex compounds. In the second step, a separation and preconcentration method for Hg determination in wine is proposed. Two sorbents were compared for the separation and preconcentration of Hg: Chelex 100 and Dowex 50W. The quantitative sorption of both Hg species was achieved by using Chelex 100 at pH 4. Quantitative elution of the Hg retained on the resin is possible with 2 mol/l HNO3. The influence of interfering factors Fe and Cu on the absorption signal is investigated, and the optimal wine amount and dilution are suggested. The limits of determination of the analytical procedure consist of Hg pre-concentration on Chelex 100 followed by CV-AAS measurement of eluted Hg, which allows the determination of total Hg in all types of wine samples with their natural contents.

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Article Details

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Vol 77 No 2 (2023): Journal of Agricultural, Food and Environmental Sciences, JAFES
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