BACILLUS SPP. – A POTENT BIOLOGICAL CONTROL AGENTS AGAINST DOWNY MILDEW OF GRAPEVINE
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Abstract
Plasmopara viticola, the causal agent of downy mildew of grapevine, is one of the most devastating and economically most important grapevine pathogens worldwide. In the Republic of North Macedonia, control of downy mildew in grapevine is mainly achieved by application of synthetic fungicides. However, the long-term and continuous use of synthetic fungicides leads to appearance of resistant strains of the pathogen, residues and environmental pollution. Alternatives to chemical control such as the use of biological control agents or resistant cultivars, currently play a small role in controlling this disease. A promising alternative strategy that could replace or be combined with fungicides are biofungicides. The main goal of the experiment was to observe the possibility for biological control of grape downy mildew by the use of two novel biofungicides, Sonata (a.m. Bacillus pumilus QST 2808) and Serenade Aso (a.m. Bacillus subtilis QST 713). Experiment was conducted during the 2019 in two vineyard regions in the Republic of North Macedonia (Demir Kapija and Negotino), on three grape varieties (Merlot, Riesling and Vranec). Both tested biofungicides showed almost equal efficacy, with more than 94% reduction of disease severity index on leaves in Vranec and Riesling varieties and with more than 97% reduction of disease severity index on leaves in Merlot variety. On bunches, both biofungicides achieved 100% reduction of disease incidence and severity index in all three varieties. The results showed that Bacillus pumilus and Bacillus subtilis are potent biological agents and have a prospective use for control of downy mildew in grapevine.
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Alfonzo, A., Lo Piccolo, S., Conigliaro, G., Ventorino, V., Burruano, S. & Moschetti, G. (2012). Antifungal peptides produced by Bacillus amyloliquefaciens AG1 active against grapevine fungal pathogens. Annual Microbiology, 62, 1593–1599.
Chen, WJ., Delmotte, F., Richard-Cervera, S., Douence, L., Greif, C. & Corio-Costet, MF. (2007). At least two origins of fungicide resistance in grapevine downy mildew populations. Appliedl Environmental Microbiology, 73, 5162–5172.
Compant, S., Brader, G., Muzammil, S., Sessitsch, A., Lebrihi, A. & Florence M. (2013). Use of beneficial bacteria and their secondary metabolites to control grapevine pathogen diseases. BioControl, 58 (4), 435-455.
Furuya, S., Mochizuki, M., Aoki, Y., Kobayashi, H., Takayanagi, T., Shimizu, M. & Suzuki S. (2011). Isolation and characterization of Bacillus subtilis KS1 for the biocontrol of grapevine fungal diseases. Biocontrol Science and technology 21 (5-6), 705-720.
Gessler, C., Pertot, I. & Perazzolli, M. (2011). Plasmopara viticola: a review of knowledge on downy mildew of grapevine and effective disease management. Phytopathologica Mediterranea, 50, 3−44.
Hamaoka, K., Aoki, Y. & Suzuki, S. (2021). Isolation and Characterization of Endophyte Bacillus velezensis KOF112 from Grapevine Shoot Xylem as Biological Control Agent for Fungal Diseases. Plants, 10, 1815.
Perazzolli, M., Moretto, M., Fontana, P., Ferrarini, A., Velasco, R., Moser, C., Delledonne, M. & Pertot, I. (2012). Downy mildew resistance induced by Trichoderma harzianum T39 in susceptible grapevines partially mimics transcriptional changes of resistant genotypes. BMC Genomics, 13, 660.
Pezzotti, G,, Fujita, Y., Boschetto, F., Zhu, W., Marin, E., Vandelle, E., McEntire, BJ., Bal, SB., Giarola, M., Makimura, K. & Polverari, A. (2020). Activity and Mechanism of Action of the Bioceramic Silicon Nitride as an Environmentally Friendly Alternative for the Control of the Grapevine Downy Mildew Pathogen Plasmopara viticola. Front. Microbiol., 11, 610211.
Pimentel, D., Acquay, H., Biltonen, M., Rice, P., Silva, M., Nelson, J., Lipner, V., Giordano, S., Horowitz, A. & D'Amore, M. (1992). Environmental and economic costs of pesticide use. Bioscience, 42, 750–760.
Santoyo, G., Orozco-Mosqueda, M.D.C. & Govindappa, M. (2012). Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: a review. Biocontrol Sci. Techn., 22, 855–872.
Townsend, G. R. & J. W. Heuberger. (1943). Methods for estimating losses caused by diseases in fungicides experiments. Plant Disease Reporter, 27, 340-343.
Romanazzi, G., Mancini, V., Feliziani, E., Servili, A., Endeshaw, S., & Neri, D. (2016). Impact of alternative fungicides on grape downy mildew control and vinegrowth and development. Plant Disease, 100, 739-748.
Li, Y., Héloir, M., Zhang, X., Geissler, M., Trouvelot, S., Jacquens, L., Henkel, M., Su, X., Fang, X., Wang, Q & Adrian, M. (2019). Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defence stimulation. Molecular Plant Pathology, 20 (8), 1037–1050.
Zang, C., Lin, Q., Xie, J., Lin, Y., Zhao, K. & Liang C. (2020). The biological control of the grapevine downy mildew disease using Ochrobactrum sp. Plant Protection Science, 56, 52–61.
Zhang, X., Zhou, Y., Li, Y., Fu, X. & Wang, Q. (2017). Screening and characterization of endophytic Bacillus for biocontrol of grapevine downy mildew. Crop Protection, 96, 173-179.