ПРИМЕНА НА РЕВЕРЗНО-ФАЗНА ВИСОКОЕФИКАСНА ТЕЧНА ХРОМАТОГРАФИЈА ЗА ОПРЕДЕЛУВАЊЕ НА АКТИВНИТЕ КОМПОНЕНТИ ВО ПРЕПАРАТОТ INTER OF APPLICATION OF REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINATION OF ACTIVE COMPONENTS IN PESTICIDE INTER OF

Abstract

Fast, simple, precise and accurate methods development for simultaneous determination of active ingredients phenmedipham, desmedipham and ethofumesate in a pesticide formulation Inter Of using reversed-phase (RP) high performance liquid chromatography (HPLC) and ultraviolet diode array detector (UV/DAD) is presented in this work. For realization the established purpose seven analytical columns are used: Hypersil ODS (25 cm  0.46 cm, 5 m), HS 3  3 C-18 (3.3 cm  0.46 cm, 3m), HS 3  3 C-8 (3.3 cm  0.46 cm, 3 m), LiChrospher 100 RP-18 (25 cm  0.4 cm, 5 m), Chromolith Performance RP-18e (10 cm x 0.46 cm), LiChrospher 60 RP-select B (12.5 cm x 0.4 cm, 5 m) and LiChrospher 60 RP-select B (25 cm x 0.4 cm, 5 m). RP-HPLC method is carried out on a LiChrospher 60 RP-select B (25 cm x 0.4 cm, 5 m) analytical column, with mobile phase of methanol/water (60/40, V/V), flow rate of 1 mL/min, constant column temperature at 25 oC, UV detection at 230 nm and 0.5 AUFS sensitivity level. The following parameters are determined for the developed method: retention factor, separation factor, limit of detection, limit of quantification, repeatability or intermediate precision of obtained results for retention time, peak area and peak height, the fit type between the mass of analyte and peak area or peak height, recovery of analyte and active ingredients quantity in a pesticide formulation.