DNA MICROSATELLITE INFORMATIVENESS, ALLELE FREQUENCIES AND THEIR DISTRIBUTION IN THE GENOME OF MACEDONIAN AUTOCHTHONOUS SHEEP POPULATIONS
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Published:
May 29, 2020
Keywords:
pramenka, sheep, DNA, microsatellites.
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Abstract
As a result of natural selection, management system, environment pressure, different populations of Pramenka sheep in South East Europe developed distinctive productive and phenotype characteristics which are sufficient to distinguish them. In this study, the informativeness of fifteen DNA microsatellite markers in the genome of 105 individuals from three indigenous sheep populations in the country (Sharplaninian, Karakachanian and Ovchepolian) was analysed. In overall population, 281 different alleles were observed. The average number of alleles per locus in total population was 18.73, the mean He in overall population was 0.824, so all loci were highly polymorphic with PIC value higher than 0.5. Mean number of alleles per locus, at population level, were 12.13, 11.53 and 13.27 alleles in the genome of Sharplaninian, Ovchepolian and Karakachanian sheep populations, respectively. Results from this research will improve knowledge of the sheep genetic resources in the country at DNA level and will provide appropriate information for further conservation.
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References
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Ocampo, R., Cardona, H., & Martínez, R. (2016). Genetic diversity of Colombian sheep by microsatellite markers. Chilean Journal of Agricultural Research, 76(1), 40-47).
Quiroz, J., Martínez, A., Landi, V., Zaragoza, L., Martínez, R., & Perezgrovas, G. (2007). Relación genética de la raza ovina de Chiapas con algunas razas ovinas españolas. Archivos de Zootecnia, 56, 441-447.
Zamani, P., Akhondi, M., & Mohammadabadi, M. R. (2015). Associations of inter-simple sequence repeat loci with predicted breeding values of body weight in sheep. Small Ruminant Res., 132, 123–127.
Arora, R., & Bhatia, S. (2006). Genetic diversity of Magra sheep from India using microsatellite analysis. Asian Australasian Journal of Animal Sciences, 19(7), 938-942.
Blackburn, H. D., Toishibekov, Y., Toishibekov, M., Welsh, C. S., Spiller, S. F., & Brown, M. (2011). Genetic diversity of Ovis aries populations near domestication centers and in the new world. Springer Genetic, 139, 1169-1178.
Cañon, J., Garćıa, D., Garćıa-Atance, M. A., Obexer-Ruff, G., Lenstra, J. A., Ajmone-Marsan, P., Dunner, S., & Econogene Consortium. (2006). Geographical partitioning of goat diversity in Europe and the Middle East. Animal Genetics, 37(4), 327–334.
Diez-Tascon, C., Littlejohn, R. P., Almeida, P. A. R., & Crawford A. M. (2000). Genetic variation within the Merino sheep breed: Analysis of closely related populations using microsatellites. Animal Genetics, 31(4), 243-251.
Efremov, G. D., Dimovski, A., Plasheska-Karanfilska, D., Shukarova, E., Koceva, S., & Popovski, Z. T. (1998). Laboratory manual. 2-nd edition MASA RCGEB.
El-Nahas Soheir, M., Hassan Amal, A., Abou Mossallam Ahlam, A., Mahfouz Eman, R., Bibars Mona, A., Oraby Hanaa, A. S., & de Hondt H. A. (2008). Analysis of genetic variation in different sheep breeds using microsatellites. African Journal of Biotechnology, 7(8), 1060-1068.
El-fawal, M. A. (2006). Molecular genetic diversity in characteristics of some Egyptian sheep breeds [Master thesis], Animal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
Ghazy, A., Mokhtar, S., Eid, M., Amin, A., Elzarei, M., Kizaki, K., & Hashizume, K. (2013). Genetic Diversity and Distances of Three Egyptian Local Sheep Breeds Using Microsatellite Markers. Research in Zoology, 3(1), 1-9. DOI: 10.5923/j.zoology.20130301.01).
Gutiérrez-Gil, B., Uzun, M., Arranz, J., Primitivo, F. S., Yildiz, S., Cenesiz, M., & Bayon, Y. (2006). Genetic diversity in Turkish sheep. Acta Agriculturae Scandinavica, Section A- Animal Science, 56(1), 1-7.
Hassan, A.A., AbouMosallam, H.A., Oraby, H. A., de Hondt, & El-Nahas, S. M. (2003). Genetic Diversity of Three Sheep Breeds in Egypt Based on Microsatellites Analysis. Journal of genetic Engineering and Biotechnology, (NRC) 1(1),141-150.
Hoda, A., & Ajmone-Marsan, P. (2012). Genetic Characterization of Albanian Sheep Breeds by Microsatellite Markers. Chapter February 2012 DOI: 10.5772/34554, Source: InTech.
Khodabakhshzadeh, R., Mohammadabadi, M. R., Esmailizadeh, A., Moradi Shahrebabak, H., Bordbar, F., & Ansari Namin, S. (2016). Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Pol. J. Vet. Sci., 19, 281–289.
Kalinowski, S.T. (2002). How many alleles per locus should be used to estimate genetic distances? Heredity, 88, 62–65.
Kim, K. S., Yeo, J. S. & Choi, C. B. (2002). Genetic diversity of north-east Asian cattle based on microsatellite data. Animal Genetics 33(3), 201-204.
Jevšinek, S. D., Šalamon, D., Džidić, A., Bojkovski, D., & Simčič, M. (2015). Genetic diversity of Bela Krajina Pramenka compared to three Croatian sheep breeds – a preliminary study Poljoprivreda/Agriculture, 21(1), 142-145. ISSN: 1848-8080 (Online) ISSN: 1330-7142 (Print) http://dx.doi.org/10.18047/poljo.21.1.sup.33)
Ochipinti, G., Núñez, L., Cazal, C., Samudio, A., Castro, L., & Ramírez, L. (2012). Diversidad genética en ovejas de las humedadesde la región Oriental del Paraguay. Actas Iberoamericanas de Conservación Animal, 2, 227-230.
Porcu, K., Markovic, B. (eds) (2006). Catalogue of West Balkan Pramenka Sheep Breed Types. Faculty of Agricultural Sciences and Food, Skopje.
Salamon, D., Gutierrez-Gil, B., Antun, K., Gorjanc, G., Kompan, D., & Dzidic, A. (2012). Preliminary study on the genetic diversity of the Istrian sheep, Lika and Krk Pramenka sheep populations using microsatellite markers. Acta agriculturae Slovenica, Supplement 3, 125–129.
Salamon, D., Gutierrez-Gil, B., Simcic, M., Kompan, D., & Dzidic, A. (2015). Microsatellite based genetic structure of regional transboundary Istrian sheep breed populations in Croatia and Slovenia. Mljekarstvo, 65(1), 39-47.
Ocampo, R., Cardona, H., & Martínez, R. (2016). Genetic diversity of Colombian sheep by microsatellite markers. Chilean Journal of Agricultural Research, 76(1), 40-47).
Quiroz, J., Martínez, A., Landi, V., Zaragoza, L., Martínez, R., & Perezgrovas, G. (2007). Relación genética de la raza ovina de Chiapas con algunas razas ovinas españolas. Archivos de Zootecnia, 56, 441-447.
Zamani, P., Akhondi, M., & Mohammadabadi, M. R. (2015). Associations of inter-simple sequence repeat loci with predicted breeding values of body weight in sheep. Small Ruminant Res., 132, 123–127.