FOLIN–CIOCÂLTEU METHOD FOR DETERMINATION OF TOTAL POLYPHENOLS IN ONION
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Апстракт
Onion is one of the most important crop in the world and the richest source of polyphenols. Polyphenols are organic compounds that are naturally present in onions and influence to its quality. The content of polyphenols depends on different factors. In this research determination of total polyphenols and flavonoids in two Macedonian local populations of onions was done by UV-Vis spectroscopy. The extraction of polyphenols from onions was performed with 60% methanol, while time for extraction was 120 minutes. Required time for completion of the reaction between polyphenols and Folin – Ciocâlteu reagent was examined. The total polyphenolic content was determined by the Folin – Ciocâlteu method with gallic acid as a reference standard. The obtained results were presented as mg GAE/100 g onion. Furthermore, the content of flavonoids in analyzed onions was determined with quercetin as a reference standard. The results were presented as mg QE/100 g onion. Polyphenols and flavonoids were determined in the onions’ extracts immediately after extraction and in the extracts stored 18 hours in a refrigerator at 4 °C. The results showed that there is no significant difference in content of polyphenols in analyzed onions, while the flavonoid content was higher in a population Melnik.
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Bahorun, T., Luximon‐Ramma, A., Crozier, A., & Aruoma, O. I. (2004). Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. Journal of the Science of Food and Agriculture, 84(12), 1553-1561.
Benkeblia, N. (2005). Free-radical scavenging capacity and antioxidant properties of some selected onions (Allium cepa L.) and garlic (Allium sativum L.) extracts. Brazilian archives of biology and technology, 48, 753-759.
Dai, J., & Mumper, R. J. (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313-7352.
Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., & Ju, Y. H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of food and drug analysis, 22(3), 296-302.
Duan, Y., Kim, M. A., Seong, J. H., Chung, H. S., & Kim, H. S. (2014). Antioxidative activities of various solvent extracts from haw (Crataegus pinnatifida Bunge). Korean Journal of Food Preservation, 21(2), 246-253.
Griffiths, G., Trueman, L., Crowther, T., Thomas, B., & Smith, B. (2002). Onions—a global benefit to health. Phytotherapy research, 16(7), 603-615.
Jozinović, A. (2015). Svojstva kukuruznih snack proizvoda obogaćenih s nusproizvodima prehrambene industrije, Doctoral dissertation, Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology, Department of Food Technologies, Sub-department of Carbohydrates Technology.
Kaur, C., Singh, M., Walia, S., Joshi, S., & Munshi, A. D. (2010). Variations in phenolics and antioxidants in Indian onions (Allium cepa L.): Genotype selection for breeding. Nutrition & Food Science, 40(1), 6-19.
Kim, J. W., Kim, Y. S., & Kyung, K. H. (2004). Inhibitory activity of essential oils of garlic and onion against bacteria and yeasts. Journal of food protection, 67(3), 499-504.
Lorrain, B., Ky, I., Pechamat, L., & Teissedre, P. L. (2013). Evolution of analysis of polyhenols from grapes, wines, and extracts. Molecules, 18(1), 1076-1100.
Lachman, J., Pronek, D., Hejtmánková, A., Dudjak, J., Pivec, V., & Faitová, K. (2003). Total polyphenol and main flavonoid antioxidants in different onion (Allium cepa L.) varieties. Horticultural science, 30(4), 142-147.
Lu, M., Yuan, B., Zeng, M., & Chen, J. (2011). Antioxidant capacity and major phenolic compounds of spices commonly consumed in China. Food Research International, 44(2), 530-536.
Nile, S. H., & Park, S. W. (2013). Total phenolics, antioxidant and xanthine oxidase inhibitory activity of three colored onions (Allium cepa L.). Frontiers in Life Science, 7(3-4), 224-228.
Pérez-Gregorio, R. M., García-Falcón, M. S., Simal-Gándara, J., Rodrigues, A. S., & Almeida, D. P. (2010). Identification and quantification of flavonoids in traditional cultivars of red and white onions at harvest. Journal of Food Composition and Analysis, 23(6), 592-598.
Pérez-Gregorio, M. R., García-Falcón, M. S., & Simal-Gándara, J. (2011). Flavonoids changes in fresh-cut onions during storage in different packaging systems. Food Chemistry, 124(2), 652-658.
Sellappan, S., & Akoh, C. C. (2002). Flavonoids and antioxidant capacity of Georgia-grown Vidalia onions. Journal of agricultural and food chemistry, 50(19), 5338-5342.
Slimestad, R., Fossen, T., & Vågen, I. M. (2007). Onions: a source of unique dietary flavonoids. Journal of agricultural and food chemistry, 55(25), 10067-10080.
Singh, B. N., Singh, B. R., Singh, R. L., Prakash, D., Singh, D. P., Sarma, B. K., ... & Singh, H. B. (2009). Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities. Food and Chemical Toxicology, 47(6), 1161-1167.
Spencer, J. P., Abd El Mohsen, M. M., Minihane, A. M., & Mathers, J. C. (2008). Biomarkers of the intake of dietary polyphenols: strengths, limitations and application in nutrition research. British Journal of Nutrition, 99(1), 12-22.
Sun, T., Powers, J. R., & Tang, J. (2007). Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food chemistry, 105(1), 101-106.
Williams, G. J., Zhang, C., & Thorson, J. S. (2007). Expanding the promiscuity of a natural-product glycosyltransferase by directed evolution. Nature chemical biology, 3(10), 657-662.
Yang, J., Meyers, K. J., van der Heide, J., & Liu, R. H. (2004). Varietal differences in phenolic content and antioxidant and antiproliferative activities of onions. Journal of agricultural and food chemistry, 52(22), 6787-6793.